Overlapping sequences are the result of several pathogens in the sample and, in the case of bacteria, could not be resolved by the sequencing primers SeqGN16 and SeqGP16. We recommend using an online tool available from Pathogenomix called RipSeq ...
The primer is used in conjunction with Mastermix 18S Complete. The primer SeqYeast18 binds to conserved sites within the 18S rRNA gene region amplified in the assays.
The Set of Eubacterial Sequencing primers contains one primer, SeqGN16, which mainly targets Gram-negative bacteria, and a second one, SeqGN16, which mainly targets Gram-positive bacteria. But both sequencing primers show exceptions, a few ...
Set of Eubacterial Sequencing Primers SeqGP16 & SeqGN16 The two primers are used in conjunction with Mastermix 16S Complete. Both primers bind to conserved sites within the 16S rRNA gene region amplified in the assays. Primer SeqGP16 is ...