Overlapping sequences are the result of several pathogens in the sample and, in the case of bacteria, could not be resolved by the sequencing primers SeqGN16 and SeqGP16. We recommend using an online tool available from Pathogenomix called RipSeq ...
Polymicrobial infection Yes, for a fee, there is an online tool available from Pathogenomix called RipSeq® which can identify up to three bacterial species from a sequence depending on the sequence quality. With the two sequencing primers, SeqGN16 ...
Sanger Sequencing Service The cost for Sanger sequencing at a 3rd party is significantly below 10€ in Germany but might differ between countries and providers.
Set of Eubacterial Sequencing Primers SeqGP16 & SeqGN16 The two primers are used in conjunction with Mastermix 16S Complete. Both primers bind to conserved sites within the 16S rRNA gene region amplified in the assays. Primer SeqGP16 is ...