For which application do I need the Set of Eubacterial Sequencing Primers?
Set of Eubacterial Sequencing Primers
SeqGP16 & SeqGN16
- The two primers are used in conjunction with Mastermix 16S Complete.
- Both primers bind to conserved sites within the 16S rRNA gene region amplified in the
assays.
- Primer SeqGP16 is
group-specific including, among others, Gram-positive Staphylococcus, and Streptococcus, Enterococcus, Listeria, Micrococcus,
Bacillus, and Lactococcus species.
- Primer SeqGN16 includes Gram-negative Pseudomonas, Acinetobacter,
Serratia, Enterobacter, Escherichia, Proteus, Klebsiella, Stenotrophomonas, and Legionella species. Using both
sequencing primers, mixed infections by bacteria in the two different groups
can be analyzed.
Related Articles
Do the primers differentiate between Gram-positive and Gram-negative bacteria?
The Set of Eubacterial Sequencing primers contains one primer, SeqGN16, which mainly targets Gram-negative bacteria, and a second one, SeqGN16, which mainly targets Gram-positive bacteria. But both sequencing primers show exceptions, a few ...
Set of Eubacterial Sequencing Primer Manuals
The latest versions of the following manuals are available for download below: Set of Eubacterial Sequencing Primer Manual (English)
Can I detect Borreliella (Borrelia) using Molzym's PCR and sequencing primers?
Amplification of Borreliella (Borrelia) with Molzym PCR primers (incl. in MA Bac and Mastermix 16S Complete) should work successfully to produce a 16S amplicon (i.e. positive/negative PCR). However, due to mismatches with our sequencing primers ...
How many sequencing reactions are needed per sample?
Sequencing For every sample with a positive 16S PCR, there are two sequencing reactions based on two sequencing primers, SeqGN16 and SeqGP16. This allows the identification of up to two pathogens, mainly separated into Gram-negative and Gram-positive ...
How can bacteria and fungi be identified when detected by 16S or 18S rDNA PCR?
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