Eubacterial sequencing primer usage

For which application do I need the Set of Eubacterial Sequencing Primers?


Set of Eubacterial Sequencing Primers
SeqGP16 & SeqGN16

  1. The two primers are used in conjunction with Mastermix 16S Complete.
  2. Both primers bind to conserved sites within the 16S rRNA gene region amplified in the assays.
  3. Primer SeqGP16 is group-specific including, among others, Gram-positive Staphylococcus, and Streptococcus, Enterococcus, Listeria, Micrococcus, Bacillus, and Lactococcus species.
  4. Primer SeqGN16 includes Gram-negative Pseudomonas, Acinetobacter, Serratia, Enterobacter, Escherichia, Proteus, Klebsiella, Stenotrophomonas, and Legionella species. Using both sequencing primers, mixed infections by bacteria in the two different groups can be analyzed.