Can pathogens be identified in mixed infections? What to do with overlapping sequences?
Overlapping
sequences
are the result of several pathogens in the sample and, in the case of
bacteria, could not be resolved by the sequencing primers
SeqGN16 and SeqGP16.
We
recommend using an online tool available from Pathogenomix called RipSeq which
can, for a fee, identify up to three bacterial species from a sequence depending on the sequence quality.
With
the two sequencing primers, SeqGN16 and SeqGP16, up to six bacterial
species can theoretically be identified from one sample.