Identification from positive amplicons The direct way is (Sanger) sequence analysis. Amplicons are purified in a simple procedure, using a commercial PCR purification kit (e.g. QIAQuick PCR Purification Kit, Qiagen, Germany). The purified amplicon is ...
Amplification of Borreliella (Borrelia) with Molzym PCR primers (incl. in MA Bac and Mastermix 16S Complete) should work successfully to produce a 16S amplicon (i.e. positive/negative PCR). However, due to mismatches with our sequencing primers ...
Set of Eubacterial Sequencing Primers SeqGP16 & SeqGN16 The two primers are used in conjunction with Mastermix 16S Complete. Both primers bind to conserved sites within the 16S rRNA gene region amplified in the assays. Primer SeqGP16 is ...