What are the differences between the kits including the MolYsis™ technology?
Please refer to the table below to
see all differences:
|
Kit name / specifications |
Sample materials
|
Processing
|
Host DNA depletion & lysis of
bacterial and fungal cells
|
Bacterial and Fungal DNA isolation
|
|
MolYsis™
Basic5 |
Fluids
<
1ml and 5 ml
|
Manual
|
Yes
|
No*
|
|
MolYsis™ Complete5 |
Fluids
< 1ml and 5 ml
|
Manual
|
Yes
|
Yes
|
|
Ultra-Deep
Microbiome Prep |
Fluids
< 1ml and tissues
|
Manual
|
Yes
|
Yes
|
|
Ultra-Deep Microbiome Prep10 |
Fluids 10 ml and tissues
|
Manual
|
Yes
|
Yes
|
|
MolYsis-SelectNA™plus |
Fluids
< 1ml, swabs, tissues
|
Fully
automated
|
Yes
|
Yes
|
* after
MolYsis™ Basic5, any kit or method for DNA isolation can be used (manual or
automated) but should include a protease or proteinase K step
Related Articles
What is MolYsis™?
MolYsis™ is a technology that lyses host (human or animal) cells and quantitatively depletes host DNA (and all other free DNA) by a DNase treatment. In the second step, intact microorganisms are lysed, and highly enriched microbial DNA is isolated. ...
How is human DNA depleted during sample processing?
Human DNA depletion Samples are treated with a buffer lysing the human/animal cells, while bacterial and fungal cells stay intact. The released human/animal DNA is enzymatically degraded along with all other free DNA and bacterial and fungal cells ...
After host DNA depletion with the MolYsis™ technology, can I isolate RNA?
RNase Please note that the MolYsis™-based kits are not systematically tested for the presence of RNases. After MolYsis™ Basic5 or step 7 of the other MolYsis™-based kits’ protocols, RNA isolation protocols could be followed.
Do I need to change my validated DNA purification system?
Not necessarily. Thanks to its modular nature, MolYsis™ Basic5 is adaptable to any DNA purification system. The kit is designed to treat <1 ml and 5 ml fluid samples including host DNA depletion and lysis of bacterial and fungal cells. Afterward, the ...
How to process tissue?
Tissue Pre-treatment Tissue is pre-treated with a 10-minute enzymatic approach followed by the normal protocol of host DNA depletion for SepsiTest™-UMD or placed into the SelectNA™plus instrument with Micro-Dx™. Other tissue treatments like bead ...