MolYsis-based kits

What are the differences between the kits including the MolYsis™ technology?

Please refer to the table below to see all differences:

Kit name / specifications

Sample materials

& volumes

Processing

Host DNA depletion & lysis of bacterial and fungal cells

Bacterial and Fungal DNA isolation

MolYsis™ Basic5

Fluids

< 1ml and 5 ml

Manual

Yes

No*

MolYsis™ Complete5

Fluids 

< 1ml and 5 ml

Manual

Yes

Yes

Ultra-Deep Microbiome Prep

Fluids < 1ml and tissues

Manual

Yes

Yes

Ultra-Deep Microbiome Prep10

Fluids 10 ml and tissues

Manual

Yes

Yes

MolYsis-SelectNA™plus

Fluids < 1ml, swabs, tissues

Fully automated

Yes

Yes

* after MolYsis™ Basic5, any kit or method for DNA isolation can be used (manual or automated) but should include a protease or proteinase K step


    • Related Articles

    • What is MolYsis™?

      MolYsis™ is a technology that lyses host (human or animal) cells and quantitatively depletes host DNA (and all other free DNA) by a DNase treatment. In the second step, intact microorganisms are lysed, and highly enriched microbial DNA is isolated. ...

    • How is human DNA depleted during sample processing?

      Human DNA depletion Samples are treated with a buffer lysing the human/animal cells, while bacterial and fungal cells stay intact. The released human/animal DNA is enzymatically degraded along with all other free DNA and bacterial and fungal cells ...

    • Do I need to change my validated DNA purification system?

      Not necessarily. Thanks to its modular nature, MolYsis™ Basic5 is adaptable to any DNA purification system. The kit is designed to treat <1 ml and 5 ml fluid samples including host DNA depletion and lysis of bacterial and fungal cells. Afterward, the ...

    • After host DNA depletion with the MolYsis™ technology, can I isolate RNA?

      RNase Please note that the MolYsis™-based kits are not systematically tested for the presence of RNases. After MolYsis™ Basic5 or step 7 of the other MolYsis™-based kits’ protocols, RNA isolation protocols could be followed.

    • Why deplete host (human or animal) DNA before PCR-based assays for bacteria and fungi?

      Why is MolYsis useful? A major problem of the direct PCR-based detection of bacteria and fungi from clinical specimens is that host DNA can exceed microbial target sequences by several thousand-fold. Host DNA contains unspecific binding sites e.g., ...