Do I need to change my validated DNA purification system?
Not necessarily.
Thanks to its
modular nature, MolYsis™ Basic5 is adaptable to any DNA purification system.
The kit is designed to treat <1 ml and 5 ml fluid samples including host DNA
depletion and lysis of bacterial and fungal cells.
Afterward, the isolation of
microbial DNA can be performed with other kits, including Qiagen, Roche, Promega,
Biomérieux, and Epicentre.
DNA isolation kit
Make sure that ingredients of DNA isolation kits of
other manufacturers are free of microbial DNA contaminations (reagents,
buffers, spin columns, plastics) and include a protease or proteinase K step.
If you are uncertain, ask your supplier.