Do I need to change my validated DNA purification system?
Not necessarily.
Thanks to its
modular nature, MolYsis™ Basic5 is adaptable to any DNA purification system.
The kit is designed to treat <1 ml and 5 ml fluid samples including host DNA
depletion and lysis of bacterial and fungal cells.
Afterward, the isolation of
microbial DNA can be performed with other kits, including Qiagen, Roche, Promega,
Biomérieux, and Epicentre.
DNA isolation kit
Make sure that ingredients of DNA isolation kits of
other manufacturers are free of microbial DNA contaminations (reagents,
buffers, spin columns, plastics) and include a protease or proteinase K step.
If you are uncertain, ask your supplier.
Related Articles
What does “DNA-free” mean?
“DNA-free” products by Molzym are defined as reagents and plastics being negative for bacterial and fungal DNA for up to 40 PCR cycles using broad-range bacterial and fungal PCR assays (Mastermix 16S Complete and Mastermix 18S Complete). Therefore, a ...Which reagents can bear contamination by microbial DNA?
In principle, all! In particular, in our experience, the Taq DNA polymerase and primers are mostly prone to DNA contamination due to the manufacturing processes. Molzym has invested great efforts to develop a purification process for the elimination ...How is the human DNA depleted?
MolYsis Technology for human DNA depletion Samples are treated with a buffer lysing the human cells, while bacterial and fungal cells stay intact. The released human DNA and other free DNA are enzymatically degraded. Afterward, the microbial cells ...Can blood cultures be used for the isolation of microbial DNA?
Blood culture All kits based on the MolYsis™ technology can be used for the isolation of microbial DNA from blood cultures. The blood culture samples shall be diluted at least 1:10 (100 µl blood culture + 900 µl SU buffer) before starting the ...What is MolYsis™?
MolYsis™ is a technology that lyses host (human or animal) cells and quantitatively depletes host DNA (and all other free DNA) by a DNase treatment. In the second step, intact microorganisms are lysed, and highly enriched microbial DNA is isolated. ...