Why is MolYsis useful for PCR assays for bacterial and fungal detection?

Why deplete host (human or animal) DNA before PCR-based assays for bacteria and fungi?

Why is MolYsis useful? 

  1. A major problem of the direct PCR-based detection of bacteria and fungi from clinical specimens is that host DNA can exceed microbial target sequences by several thousand-fold.
  2. Host DNA contains unspecific binding sites e.g., 16S primers leading to unspecific binding which limits the accessibility of primers to the specific sites on the 16S rRNA gene and may lead to false-positive signals. 
  3. The depletion of host DNA increases the sensitivity and specificity of the detection of bacterial and fungal DNA from direct samples.




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