After host DNA depletion with the MolYsis™ technology, can I isolate RNA?

RNase

Please note that the MolYsis™-based kits are not systematically tested for the presence of RNases.

After MolYsis™ Basic5 or step 7 of the other MolYsis™-based kits’ protocols, RNA isolation protocols could be followed. 

• Related Articles

• What are the differences between the kits including the MolYsis™ technology?

  Please refer to the table below to see all differences: Kit name / specifications Sample materials & volumes Processing Host DNA depletion & lysis of bacterial and fungal cells Bacterial and Fungal DNA isolation MolYsis™ Basic5 Fluids < 1ml and 5 ml ...

• What is MolYsis™?

  MolYsis™ is a technology that lyses host (human or animal) cells and quantitatively depletes host DNA (and all other free DNA) by a DNase treatment. In the second step, intact microorganisms are lysed, and highly enriched microbial DNA is isolated. ...

• Is the MolYsis™ technology applicable for viruses/viral NA isolation?

  The MolYsis™ technology has not been systematically tested for the isolation of viral NA.

• Is the MolYsis™ technology applicable to plant samples?

  The MolYsis™ technology has not been systematically tested for plant samples and, taking the structural properties of plant cells into account, the successful host DNA depletion of the plant DNA does not seem likely.

• How is human DNA depleted during sample processing?

  Human DNA depletion Samples are treated with a buffer lysing the human/animal cells, while bacterial and fungal cells stay intact. The released human/animal DNA is enzymatically degraded along with all other free DNA and bacterial and fungal cells ...