After host DNA depletion with the MolYsis™ technology, can I isolate RNA?

RNase

Please note that the MolYsis™-based kits are not systematically tested for the presence of RNases.

After MolYsis™ Basic5 or step 7 of the other MolYsis™-based kits’ protocols, RNA isolation protocols could be followed. 

• Related Articles

• What are the differences between the kits including the MolYsis™ technology?

  Please refer to the table below to see all differences: Kit name / specifications Sample materials & volumes Processing Host DNA depletion & lysis of bacterial and fungal cells Bacterial and Fungal DNA isolation MolYsis™ Basic5 Fluids < 1ml and 5 ml ...

• What is MolYsis™?

  MolYsis™ is a technology that lyses host (human or animal) cells and quantitatively depletes host DNA (and all other free DNA) by a DNase treatment. In the second step, intact microorganisms are lysed, and highly enriched microbial DNA is isolated. ...

• Is the MolYsis™ technology applicable for viruses/viral NA isolation?

  The MolYsis™ technology has not been systematically tested for the isolation of viral NA.

• How is human DNA depleted during sample processing?

  Human DNA depletion Samples are treated with a buffer lysing the human/animal cells, while bacterial and fungal cells stay intact. The released human/animal DNA is enzymatically degraded along with all other free DNA and bacterial and fungal cells ...

• Why deplete host (human or animal) DNA before PCR-based assays for bacteria and fungi?

  Why is MolYsis useful? A major problem of the direct PCR-based detection of bacteria and fungi from clinical specimens is that host DNA can exceed microbial target sequences by several thousand-fold. Host DNA contains unspecific binding sites e.g., ...