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How is human DNA depleted during sample processing?
Human DNA depletion Samples are treated with a buffer lysing the human/animal cells, while bacterial and fungal cells stay intact. The released human/animal DNA is enzymatically degraded along with all other free DNA and bacterial and fungal cells ...
What are the differences between the kits including the MolYsis™ technology?
Please refer to the table below to see all differences: Kit name / specifications Sample materials & volumes Processing Host DNA depletion & lysis of bacterial and fungal cells Bacterial and Fungal DNA isolation MolYsis™ Basic5 Fluids < 1ml and 5 ml ...
How is the human DNA depleted?
MolYsis Technology for human DNA depletion Samples are treated with a buffer lysing the human cells, while bacterial and fungal cells stay intact. The released human DNA and other free DNA are enzymatically degraded. Afterward, the microbial cells ...
Do I need to change my validated DNA purification system?
Not necessarily. Thanks to its modular nature, MolYsis™ Basic5 is adaptable to any DNA purification system. The kit is designed to treat <1 ml and 5 ml fluid samples including host DNA depletion and lysis of bacterial and fungal cells. Afterward, the ...
Why deplete host (human or animal) DNA before PCR-based assays for bacteria and fungi?
Why is MolYsis useful? A major problem of the direct PCR-based detection of bacteria and fungi from clinical specimens is that host DNA can exceed microbial target sequences by several thousand-fold. Host DNA contains unspecific binding sites e.g., ...