Which samples can be used for SepsiTest™-UMD?
SepsiTest-UMD & Clinical samples
For SepsiTest™-UMD the following clinical samples are
applicable:
- Body fluids: e.g., ascites, broncho-alveolar lavage, whole blood (EDTA-
or citrate-stabilized), CSF, pleural fluid, pus, synovial fluid
- Tissues: e.g., abscesses, biopsies, heart valves, prostheses,
stent
- Swabs: e.g., mouth,
nasopharynx, wounds, bones
- In general, SepsiTest™-UMD is designed for the analysis of
primary sterile samples. Therefore, feces, skin washes, and all samples with a
natural microbiome are not validated.
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Which samples can be used for Micro-Dx™?
Micro-Dx & Clinical sample For Micro-Dx™ the following clinical samples are applicable: Body fluids: e.g., whole blood (EDTA- or citrate-stabilized), pleural fluid, cerebrospinal fluid, ascites aspirates, broncho-alveolar lavage, blood cultures, ...
Which sample sizes can be used?
Clinical Sample Size & Volume For SepsiTest™-UMD and Micro-Dx™: Fluid samples: 1 ml. We recommend using as much sample volume as possible. For samples with smaller volumes, a buffer for volume adjustment to 1 ml is included in the kits. Tissue ...
What are the sample requirements? How shall samples be stored after sample collection? How fresh or old can samples be?
Please note: For any MolYsis™-based kits the microbial cells need to be intact! After taking the samples, please cool the samples in the fridge at ~8°C for short-term storage. Optimally, fresh samples are used for the highest detection sensitivity. ...
Can FFPE samples be analyzed with SepsiTest™-UMD or Micro-Dx™?
FFPE samples Yes, formalin-fixed paraffin-embedded (FFPE) samples can be processed by simply following the tissue protocol of the kit. There are no additional steps like a deparaffinization required.
Can blood culture samples be used?
Blood culture samples Yes, blood culture samples can be used but they first need to be diluted for use with SepsiTest™-UMD and Micro-Dx™. Please use 100 µl blood culture and 900 µl SU buffer for both kits. For Micro-Dx™, please ensure that the medium ...