Which samples can be used for SepsiTest™-UMD?
SepsiTest-UMD & Clinical samples
For SepsiTest™-UMD the following clinical samples are
applicable:
- Body fluids: e.g., ascites, broncho-alveolar lavage, whole blood (EDTA-
or citrate-stabilized), CSF, pleural fluid, pus, synovial fluid
- Tissues: e.g., abscesses, biopsies, heart valves, prostheses,
stent
- Swabs: e.g., mouth,
nasopharynx, wounds, bones
- In general, SepsiTest™-UMD is designed for the analysis of
primary sterile samples. Therefore, feces, skin washes, and all samples with a
natural microbiome are not validated.
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Which samples can be used for Micro-Dx™?
Micro-Dx & Clinical sample For Micro-Dx™ the following clinical samples are applicable: Body fluids: e.g., whole blood (EDTA- or citrate-stabilized), pleural fluid, cerebrospinal fluid, ascites aspirates, broncho-alveolar lavage, blood cultures, ...
Which sample sizes can be used?
Clinical Sample Size & Volume For SepsiTest™-UMD and Micro-Dx™: Fluid samples: 1 ml. We recommend using as much sample volume as possible. For samples with smaller volumes, a buffer for volume adjustment to 1 ml is included in the kits. Tissue ...
Can FFPE samples be analyzed with SepsiTest™-UMD or Micro-Dx™?
FFPE samples Yes, FFPE samples can be processed by simply following the tissue protocol of the kit. There are no additional steps like a deparaffinization required.
What are the sample requirements? How shall samples be stored after sample collection? How fresh or old can samples be?
Please note: For any MolYsis™-based kits the microbial cells need to be intact! After taking the samples, please cool the samples in the fridge at ~8°C for short-term storage. Optimally, fresh samples are used for the highest detection sensitivity. ...
Can blood culture samples be used?
Blood culture samples Yes, blood culture samples can be used but they first need to be diluted for use with SepsiTest™-UMD and Micro-Dx™. Please use 100 µl blood culture and 900 µl SU buffer for both kits. For Micro-Dx™, please ensure that the medium ...