What samples can be analysed with MolYsis kits?

What kind of samples can be processed with MolYsis™?

MolYsis™ has proven valuable for the analysis of a wide range of sample materials including various tissue specimens, body fluids, and swabs, especially for applications in the field of NGS and PCR based detection methods.

Samples evaluated for MolYsis™ Basic5 and MolYsis™ Complete5

  1. Human origin: Whole blood, synovial fluid, pleural fluid, cerebrospinal fluid, ascites fluid, pus, broncho-alveolar lavage, nasal wash fluid, urine
  2. Animal origin (please refer to “Overview on animal-derived samples” for publications): Whole blood from mouse, rat, and monkey, Chinese hamster ovary cell culture, monkey renal cell culture

Sample evaluated for Ultra-Deep Microbiome Prep and Ultra-Deep Microbiome Prep10
  1. from either human or animal origin (see "Overview on animal-derived samples" for more details):
  1. Body fluids: ascites, BAL, blood, CSF, pleural fluid, pus, synovial fluid
  2. Tissues: abscesses, biopsies, heart valves, prostheses, stents

The kits are not validated for feces/stool samples. 
  1. Feces/stool contains a high load of inhibitors which would require a high dilution for processing.


Overview of animal-derived samples including references:

Animal

Sample type(s)

Kit used

Reference(s)

DOI

Mosquitoes

Whole mosquitoes

MolYsis™ Complete5

Dada et al. 2018

10.1038/s41598-018-20367-4

Rabbits

Sacculus rotundus

 

Sacculus rotundus + vermiform appendix

Ultra-Deep Microbiome Prep

Arrazuria et al. 2016

 

Arrazuria et al. 2018

10.3389/fmicb.2016.00446

 

10.1038/s41598-018-32484-1

Dogs/ Canine

EDTA blood

MolYsis™ Complete5

McCann et Jordan 2014

10.1016/j.mimet.2014.01.013

Pigs

Ear tissue

Ultra-Deep Microbiome Prep

Costa et al. 2021

10.1016/j.vetmic.2021.109246

Cows/ Bovine

Milk

MolYsis™ Basic5

Rubiola et al. 2020

10.3389/fmicb.2020.01983

Mice

Tissue

Ultra-Deep Microbiome Prep

Martínez-López 2019

10.1016/j.immuni.2018.12.020


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