What kind of samples can be processed with MolYsis™?
MolYsis™ has
proven valuable for the analysis of a wide range of sample materials including
various tissue specimens, body fluids, and swabs, especially for applications
in the field of NGS and PCR based detection methods.
Samples evaluated for MolYsis™ Basic5 and MolYsis™ Complete5
- Human
origin: Whole blood, synovial fluid, pleural fluid, cerebrospinal fluid, ascites fluid,
pus, broncho-alveolar lavage, nasal wash fluid, urine
- Animal origin (please
refer to “Overview on animal-derived samples” for publications): Whole blood from mouse, rat, and monkey,
Chinese hamster ovary cell culture, monkey renal cell culture
Sample evaluated for Ultra-Deep Microbiome Prep and Ultra-Deep Microbiome Prep10
- from either human or animal origin (see "Overview on animal-derived samples" for more details):
- Body fluids: ascites, BAL, blood, CSF, pleural fluid, pus, synovial
fluid
- Tissues: abscesses, biopsies, heart valves, prostheses, stents
The kits are not validated for feces/stool samples.
- Feces/stool contains a high load of
inhibitors which would require a high dilution for processing.
Overview of animal-derived
samples including references:
Animal | Sample
type(s) | Kit
used | Reference(s) | DOI |
Mosquitoes | Whole mosquitoes | MolYsis™ Complete5 | Dada et al. 2018 | 10.1038/s41598-018-20367-4 |
Rabbits | Sacculus
rotundus
Sacculus
rotundus + vermiform appendix | Ultra-Deep
Microbiome Prep | Arrazuria
et al. 2016
Arrazuria
et al. 2018 | 10.3389/fmicb.2016.00446
10.1038/s41598-018-32484-1 |
Dogs/ Canine | EDTA blood | MolYsis™ Complete5 | McCann et Jordan 2014 | 10.1016/j.mimet.2014.01.013 |
Pigs | Ear
tissue | Ultra-Deep
Microbiome Prep | Costa
et al. 2021 | 10.1016/j.vetmic.2021.109246 |
Cows/ Bovine | Milk | MolYsis™ Basic5 | Rubiola et al. 2020 | 10.3389/fmicb.2020.01983 |
Mice | Tissue | Ultra-Deep
Microbiome Prep | Martínez-López
2019 | 10.1016/j.immuni.2018.12.020 |