What kind of samples can be processed with MolYsis™?

MolYsis™ has proven valuable for the analysis of a wide range of sample materials including various tissue specimens, body fluids, and swabs, especially for applications in the field of NGS and PCR based detection methods.

Samples evaluated for MolYsis™ Basic5 and MolYsis™ Complete5

1. Human origin: Whole blood, synovial fluid, pleural fluid, cerebrospinal fluid, ascites fluid, pus, broncho-alveolar lavage, nasal wash fluid, urine
   
2. Animal origin (please refer to “Overview on animal-derived samples” for publications): Whole blood from mouse, rat, and monkey, Chinese hamster ovary cell culture, monkey renal cell culture
   

Sample evaluated for Ultra-Deep Microbiome Prep and Ultra-Deep Microbiome Prep10

1. from either human or animal origin (see "Overview on animal-derived samples" for more details):
   

1. Body fluids: ascites, BAL, blood, CSF, pleural fluid, pus, synovial fluid
   
2. Tissues: abscesses, biopsies, heart valves, prostheses, stents
   

The kits are not validated for feces/stool samples. 

1. Feces/stool contains a high load of inhibitors which would require a high dilution for processing.
   

Overview of animal-derived samples including references:

Animal	Sample type(s)	Kit used	Reference(s)	DOI
Mosquitoes	Whole mosquitoes	MolYsis™ Complete5	Dada et al. 2018	10.1038/s41598-018-20367-4
Rabbits	Sacculus rotundus   Sacculus rotundus + vermiform appendix	Ultra-Deep Microbiome Prep	Arrazuria et al. 2016   Arrazuria et al. 2018	10.3389/fmicb.2016.00446   10.1038/s41598-018-32484-1
Dogs/ Canine	EDTA blood	MolYsis™ Complete5	McCann et Jordan 2014	10.1016/j.mimet.2014.01.013
Pigs	Ear tissue	Ultra-Deep Microbiome Prep	Costa et al. 2021	10.1016/j.vetmic.2021.109246
Cows/ Bovine	Milk	MolYsis™ Basic5	Rubiola et al. 2020	10.3389/fmicb.2020.01983
Mice	Tissue	Ultra-Deep Microbiome Prep	Martínez-López 2019	10.1016/j.immuni.2018.12.020

Please also refer to “What are the sample requirements?”.

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