What kind of samples can be processed with MolYsis™?
MolYsis™ has
proven valuable for the analysis of a wide range of sample materials including
various tissue specimens, body fluids, and swabs, especially for applications
in the field of NGS and PCR based detection methods.
Samples evaluated for MolYsis™ Basic5 and MolYsis™ Complete5
- Human
origin: Whole blood, synovial fluid, pleural fluid, cerebrospinal fluid, ascites fluid,
pus, broncho-alveolar lavage, nasal wash fluid, urine
- Animal origin (please
refer to “Overview on animal-derived samples” for publications): Whole blood from mouse, rat, and monkey,
Chinese hamster ovary cell culture, monkey renal cell culture
Sample evaluated for Ultra-Deep Microbiome Prep and Ultra-Deep Microbiome Prep10
- from either human or animal origin (see "Overview on animal-derived samples" for more details):
- Body fluids: ascites, BAL, blood, CSF, pleural fluid, pus, synovial
fluid
- Tissues: abscesses, biopsies, heart valves, prostheses, stents
The kits are not validated for feces/stool samples.
- Feces/stool contains a high load of
inhibitors which would require a high dilution for processing.
Overview of animal-derived
samples including references:
Animal
| Sample
type(s)
| Kit
used
| Reference(s)
| DOI
|
Mosquitoes
|
Whole mosquitoes
|
MolYsis™ Complete5
|
Dada et al. 2018
|
10.1038/s41598-018-20367-4
|
Rabbits
|
Sacculus
rotundus
Sacculus
rotundus + vermiform appendix
|
Ultra-Deep
Microbiome Prep
|
Arrazuria
et al. 2016
Arrazuria
et al. 2018
|
10.3389/fmicb.2016.00446
10.1038/s41598-018-32484-1
|
Dogs/ Canine
|
EDTA blood
|
MolYsis™ Complete5
|
McCann et Jordan 2014
|
10.1016/j.mimet.2014.01.013
|
Pigs
|
Ear
tissue
|
Ultra-Deep
Microbiome Prep
|
Costa
et al. 2021
|
10.1016/j.vetmic.2021.109246
|
Cows/ Bovine
|
Milk
|
MolYsis™ Basic5
|
Rubiola et al. 2020
|
10.3389/fmicb.2020.01983
|
Mice
|
Tissue
|
Ultra-Deep
Microbiome Prep
|
Martínez-López
2019
|
10.1016/j.immuni.2018.12.020
|
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Ultra-Deep Microbiome Prep & Ultra-Deep Microbiome Prep10 RuO Manuals
The latest versions of the following manuals are available for download below: Ultra-Deep Microbiome Prep RuO Manual (English) Ultra-Deep Microbiome Prep RuO Short Manual (English) Ultra-Deep Microbiome Prep10 RuO Manual (English) Ultra-Deep ...
Can FFPE samples be analyzed with MolYsis™-based kits?
FFPE Sample Yes, FFPE samples can be processed by simply following the tissue protocol of the Ultra-Deep Microbiome Prep and Ultra-Deep Microbiome Prep10 kits. There are no additional steps like deparaffinization required.
What are the differences between the kits including the MolYsis™ technology?
Please refer to the table below to see all differences: Kit name / specifications Sample materials & volumes Processing Host DNA depletion & lysis of bacterial and fungal cells Bacterial and Fungal DNA isolation MolYsis™ Basic5 Fluids < 1ml and 5 ml ...
Which sample sizes can be used?
Clinical Sample Size & Volume For SepsiTest™-UMD and Micro-Dx™: Fluid samples: 1 ml. We recommend using as much sample volume as possible. For samples with smaller volumes, a buffer for volume adjustment to 1 ml is included in the kits. Tissue ...
Interesting Publications
Gyarmati et al. (2015): A study with 130 blood samples from hematological patients receiving dose-intensive antitumoral treatment were analyzed using MolYsis™ Complete5 and NGS sequencing. Link Thoendel et al. (2016): Previously negative sonicate ...