Which real-time PCR cyclers are validated for SepsiTest™-UMD and Micro-Dx™?
PCR Cyclers
The
following cyclers are validated:
- LightCycler® 1.5, 2.0, 96, 480 and Nano, Roche
- DNA Engine Opticon®, CFX96™, BioRad
- Mx3000P®, Mx3005P®, Stratagene
- ABI 7500 Fast®, Life Technologies
- Rotor-Gene®, Qiagen
- peqStar 96Q, peqlab
- Not suitable are: Smartcycler®, and Cepheid®.
- If
other cyclers shall be used, the performance of all assays, MA Bac, MA Yeasts, and MA Control need to perform correctly which can be tested by following the instructions in
the manual.
Related Articles
How to interpret the PCR results?
PCR results Interpretation Amplicons can either be analyzed by conventional gel electrophoresis with bands displaying the size of the amplicons (460bp for the MA Bac and 310bp for the MA Yeasts). Alternatively, a real-time PCR cycler can be used, and ...
Can MolTaq 16S/18S and master mixes be used for Real-Time PCR?
MolTaq 16S/18S & real-time PCR Yes. All DNA-free PCR products of Molzym are fully compatible and optimized for Real-Time PCR, using fluorescent dye or probe detection systems.
How to interpret the PCR results?
Amplicons can either be analyzed by conventional gel electrophoresis with bands displaying at the size of the amplicons (460bp for the Mastermix 16S Complete and 310bp for the Mastermix 18S Complete). Alternatively, a real-time PCR cycler can be ...
Which samples can be used for Micro-Dx™?
Micro-Dx & Clinical sample For Micro-Dx™ the following clinical samples are applicable: Body fluids: e.g., whole blood (EDTA- or citrate-stabilized), pleural fluid, cerebrospinal fluid, ascites aspirates, broncho-alveolar lavage, blood cultures, ...
Micro-Dx™ CE IVD Manuals
The latest versions of the following manuals are available for download below: Micro-Dx™ CE IVD Manual (English) Micro-Dx™ CE IVD Short Manual (English) Micro-Dx™ CE IVD Short Manual (German)