What possible analysis with the eluted DNA?

Which applications are possible with the eluted DNA (after MolYsis™ technology)?

The eluted DNA can be used for diverse analyses

For example:
  1. broad-range PCR and Sanger sequencing,
  2. specific PCR assays,
  3. hybridization assays,
  4. multiplex PCR, panel systems,
  5. custom PCR assay,
  6. NGS metagenomic/shotgun sequencing,
  7. whole genome sequencing,
  8. amplicon/target sequencing.

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    • How can the eluted DNA concentration be measured?

      Diverse approaches are possible, but please be aware that due to the depletion of human/animal DNA, the DNA concentration might be quite low (between ng and fg amounts of DNA are isolated depending on the sample used). Possible methods include: qPCR ...

    • Interesting Publications

      Gyarmati et al. (2015): A study with 130 blood samples from hematological patients receiving dose-intensive antitumoral treatment were analyzed using MolYsis™ Complete5 and NGS sequencing. Link Thoendel et al. (2016): Previously negative sonicate ...

    • Is the MolYsis™ technology applicable to plant samples?

      The MolYsis™ technology has not been systematically tested for plant samples and, taking the structural properties of plant cells into account, the successful host DNA depletion of the plant DNA does not seem likely.

    • After host DNA depletion with the MolYsis™ technology, can I isolate RNA?

      RNase Please note that the MolYsis™-based kits are not systematically tested for the presence of RNases. After MolYsis™ Basic5 or step 7 of the other MolYsis™-based kits’ protocols, RNA isolation protocols could be followed.

    • Can I use Molzym's PCR assays for quantitative PCR (qPCR) analysis?

      Products: Mastermix 16S Complete, Mastermix 18S Complete, PCR assays incl. in SepsiTest™-UMD, Micro-Dx™ and UMD-SelectNA™ No. Due to the broad-range primers (16S/18S) used, potential primer dimer formation and unspecific annealling can occur, so we ...