What to do when there is no thermomixer in my lab for the MolYsis™-based kits?

MolYsis & Thermomixer

The MolYsis™ technology requires certain temperatures, 37°C, 56°C, and 70°C.

During the incubation, samples should be mixed well.

No Thermomixer? 

If no thermomixer with a shaking function is available, a heating block could be adjusted to the required temperatures, and the samples vortexed in between the incubation period.

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• After host DNA depletion with the MolYsis™ technology, can I isolate RNA?

  RNase Please note that the MolYsis™-based kits are not systematically tested for the presence of RNases. After MolYsis™ Basic5 or step 7 of the other MolYsis™-based kits’ protocols, RNA isolation protocols could be followed.

• What are the differences between the kits including the MolYsis™ technology?

  Please refer to the table below to see all differences: Kit name / specifications Sample materials & volumes Processing Host DNA depletion & lysis of bacterial and fungal cells Bacterial and Fungal DNA isolation MolYsis™ Basic5 Fluids < 1ml and 5 ml ...

• Is the MolYsis™ technology applicable for viruses/viral NA isolation?

  The MolYsis™ technology has not been systematically tested for the isolation of viral NA.

• Is the MolYsis™ technology applicable to plant samples?

  The MolYsis™ technology has not been systematically tested for plant samples and, taking the structural properties of plant cells into account, the successful host DNA depletion of the plant DNA does not seem likely.

• Can FFPE samples be analyzed with MolYsis™-based kits?

  FFPE Sample Yes, FFPE samples can be processed by simply following the tissue protocol of the Ultra-Deep Microbiome Prep and Ultra-Deep Microbiome Prep10 kits. There are no additional steps like deparaffinization required.