What to do when there is no thermomixer in my lab for the MolYsis™-based kits?
MolYsis & Thermomixer
The
MolYsis™ technology requires certain temperatures, 37°C, 56°C, and 70°C.
During
the incubation, samples should be mixed well.
No Thermomixer?
If no thermomixer with a shaking
function is available, a heating block could be adjusted to the required
temperatures, and the samples vortexed in between the incubation period.
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After host DNA depletion with the MolYsis™ technology, can I isolate RNA?
RNase Please note that the MolYsis™-based kits are not systematically tested for the presence of RNases. After MolYsis™ Basic5 or step 7 of the other MolYsis™-based kits’ protocols, RNA isolation protocols could be followed.
What are the differences between the kits including the MolYsis™ technology?
Please refer to the table below to see all differences: Kit name / specifications Sample materials & volumes Processing Host DNA depletion & lysis of bacterial and fungal cells Bacterial and Fungal DNA isolation MolYsis™ Basic5 Fluids < 1ml and 5 ml ...
Is the MolYsis™ technology applicable for viruses/viral NA isolation?
The MolYsis™ technology has not been systematically tested for the isolation of viral NA.
Is the MolYsis™ technology applicable to plant samples?
The MolYsis™ technology has not been systematically tested for plant samples and, taking the structural properties of plant cells into account, the successful host DNA depletion of the plant DNA does not seem likely.
What to do when there is no centrifuge in my lab that can reach 9,500xg for 50 ml tubes (related to MolYsis™ Basic5, MolYsis™ Complete5, or Ultra-Deep Microbiome Prep10 kit)?
No centrifuge for 9500xg/50ml tubes If your lab is not equipped with a centrifuge that can reach 9500xg for 50ml tubes*, you can use a lower speed (e.g., 4,500xg) and should extend the centrifugation time to 20 min instead of the 10 min in the ...