What to do when there is no centrifuge in my lab that can reach 9,500xg for 50 ml tubes (related to MolYsis™ Basic5, MolYsis™ Complete5, or Ultra-Deep Microbiome Prep10 kit)?

No centrifuge for 9500xg/50ml tubes

If your lab is not equipped with a centrifuge that can reach 9500xg for 50ml tubes*,

you can use a lower speed (e.g., 4,500xg) and should extend the centrifugation time to 20 min instead of the 10 min in the original protocol.

*related to MolYsis™ Basic5, MolYsis™ Complete5, or Ultra-Deep Microbiome Prep10 kits.

• Related Articles

• Ultra-Deep Microbiome Prep & Ultra-Deep Microbiome Prep10 RuO Manuals

  The latest versions of the following manuals are available for download below: Ultra-Deep Microbiome Prep RuO Manual (English) Ultra-Deep Microbiome Prep RuO Short Manual (English) Ultra-Deep Microbiome Prep10 RuO Manual (English) Ultra-Deep ...

• What kind of samples can be processed with MolYsis™?

  MolYsis™ has proven valuable for the analysis of a wide range of sample materials including various tissue specimens, body fluids, and swabs, especially for applications in the field of NGS and PCR based detection methods. Samples evaluated for ...

• Can I change the elution volume?

  For all manual RUO kits (MolYsis™ Basic5, MolYsis™ Complete5, Ultra-Deep Microbiome Prep, and Ultra-Deep Microbiome Prep10) the elution volume might be changed. A change of the elution volume is not possible with the automated MolYsis-SelectNA™plus ...

• Can FFPE samples be analyzed with MolYsis™-based kits?

  FFPE Sample Yes, FFPE samples can be processed by simply following the tissue protocol of the Ultra-Deep Microbiome Prep and Ultra-Deep Microbiome Prep10 kits. There are no additional steps like deparaffinization required.

• Interesting Publications

  Gyarmati et al. (2015): A study with 130 blood samples from hematological patients receiving dose-intensive antitumoral treatment were analyzed using MolYsis™ Complete5 and NGS sequencing. Link Thoendel et al. (2016): Previously negative sonicate ...