
Sensitivity of detection / Bacteria & Fungi
Spiking experiments showed that bacteria and fungi can be detected at low titers in whole blood using real-time
PCR and subsequent Sanger Sequencing.
For instance, with 5 ml whole blood using
MolYsis™ Complete5 and a universal 16S PCR assay (Mastermix 16S Complete) or a
universal 18S assay (Mastermix 18S Complete), the detection limits are (100%; 6
parallel determinations):