What is the sensitivity of detection of bacteria and fungi in blood?
Sensitivity of detection / Bacteria & Fungi
Spiking experiments showed that bacteria and fungi can be detected at low titers in whole blood using real-time
PCR and subsequent Sanger Sequencing.
​For instance, with 5 ml whole blood using
MolYsis™ Complete5 and a universal 16S PCR assay (Mastermix 16S Complete) or a
universal 18S assay (Mastermix 18S Complete), the detection limits are (100%; 6
parallel determinations):
- Staphylococcus
aureus, 12 CFU/ml;
- Escherichia coli, 24 CFU/ml;
- Candida albicans, 5 CFU/ml.
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