What are the differences between SepsiTest™-UMD and Micro-Dx™?
Both are culture-independent
diagnostic kits for the detection and identification of bacteria and fungi from
clinical samples by 16S and 18S rRNA amplification and subsequent Sanger
sequencing.
The difference between both kits is that the host DNA depletion and
microbial DNA isolation are performed manually with the SepsiTest™-UMD and
fully automated with the Micro-Dx™ kit. For the automated DNA extraction, the
SelectNA™plus instrument is required.
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What is included in the kits SepsiTest™-UMD and Micro-Dx™?
SepsiTest(tm)-UMD Micro-Dx(tm) Manual and short manual Yes Yes Reagents for sample pre-treatment e.g. tissue biopsies Yes Yes Reagents for host DNA depletion and microbial DNA isolation Buffers and enzymes Prefilled buffer cartridges and enzyme vials ...
Can FFPE samples be analyzed with SepsiTest™-UMD or Micro-Dx™?
FFPE samples Yes, formalin-fixed paraffin-embedded (FFPE) samples can be processed by simply following the tissue protocol of the kit. There are no additional steps like a deparaffinization required.
What IT infrastructure is required for SepsiTest™-UMD and Micro-Dx™?
IT infrastructure The required IT infrastructure is quite small: a software to view (and if required edit) sequences, e.g., Chromas internet access for the BLAST search space on the server is quite small, sequencing files are between 200 and 300 kb ...
Which part of SepsiTest™-UMD or Micro-Dx™ is CE IVD marked?
CE IVD In brief, from sample to PCR result which resembles the pathogen detection is marked CE IVD. The identification of the pathogens is not included.
Can molds be isolated and detected with SepsiTest™-UMD or Micro-Dx™?
Mold? Yes, some genera belonging to molds have been successfully isolated and detected (18S PCR), for example, Aspergillus, Fusarium, and Cladosporium. See List of Microorganisms/Fungi