What are the benefits of SepsiTest™ BLAST?
SepsiTest BLAST
SepsiTest™ BLAST is a quality-controlled
database with:
- 7,042 full-length 16S rRNA gene sequences, exclusively from
cultivated reference strains of the described species of the domains Bacteria
and Archaea
- 342 selected 18S rRNA gene sequences (Eukarya) of the
cultivated members of the genera Candida, Aspergillus, and Cryptococcus within
the Fungi
In addition, SepsiTest™ BLAST will
tell you if the quality of the sequence is not sufficient (<200 bases and/or
>5% ambiguities).
For sequences in the ABI format, also the ends will be
checked and trimmed if required (removal of N's and bases with low-quality
values).
In case the sequence quality is good, but there is no result given by
SepsiTest™ BLAST, please use an alternative database since not all species or genera
are included in SepsiTest™ BLAST.
Related Articles
How can bacteria and fungi be identified when detected by 16S or 18S rDNA PCR?
Identification from positive amplicons The direct way is (Sanger) sequence analysis. Amplicons are purified in a simple procedure, using a commercial PCR purification kit (e.g. QIAQuick PCR Purification Kit, Qiagen, Germany). The purified amplicon is ...
Can pathogens be identified in mixed infections? What to do with overlapping sequences?
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How many sequencing reactions are needed per sample?
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What is the sensitivity of detection of bacteria and fungi in blood?
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MicroDx™ CE IVD Product Flyers
The latest PDF versions of the following Flyers are available for download below: MicroDx™ CE IVD (English) a brief introduction to product features and visual overview of workflow and applications MicroDx™ CE IVD (German) eine kurze Einführung in ...