The melt curve analysis shows high primer dimer formation: why and what can I do?

Primer Dimer

Primer dimer formations are non-specific products and can be minimized by constant cooling during PCR setup.

It is completely normal that in negative samples, for example, the negative control (NC), the primer dimer can be higher compared to positive samples, for example, the positive control 1 (P1).

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• The melt curve analysis shows a high primer dimer formation: why and what can I do?

  Primer dimer Primer dimer formations are non-specific products and can be minimized by constant cooling during PCR setup. It is completely normal that in negative samples, for example, the negative control (NC), the primer dimer can be higher ...

• How to interpret the PCR results?

  Amplicons can either be analyzed by conventional gel electrophoresis with bands displaying at the size of the amplicons (460bp for the Mastermix 16S Complete and 310bp for the Mastermix 18S Complete). Alternatively, a real-time PCR cycler can be ...

• How to interpret the PCR results?

  PCR results Interpretation Amplicons can either be analyzed by conventional gel electrophoresis with bands displaying the size of the amplicons (460bp for the MA Bac and 310bp for the MA Yeasts). Alternatively, a real-time PCR cycler can be used, and ...

• Can I use Molzym's PCR assays for quantitative PCR (qPCR) analysis?

  Products: Mastermix 16S Complete, Mastermix 18S Complete, PCR assays incl. in SepsiTest™-UMD, Micro-Dx™ and UMD-SelectNA™ No. Due to the broad-range primers (16S/18S) used, potential primer dimer formation and unspecific annealling can occur, so we ...

• Can MolTaq 16S/18S and master mixes be used for Real-Time PCR?

  MolTaq 16S/18S & real-time PCR Yes. All DNA-free PCR products of Molzym are fully compatible and optimized for Real-Time PCR, using fluorescent dye or probe detection systems.