The melt curve analysis shows a high primer dimer formation: why and what can I do?

Primer dimer

Primer dimer formations are non-specific products and can be minimized by constant cooling during PCR setup.

It is completely normal that in negative samples, for example, the negative control (NC), the primer dimer can be higher compared to positive samples, for example, the positive control 1 (P1).

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• The melt curve analysis shows high primer dimer formation: why and what can I do?

  Primer Dimer Primer dimer formations are non-specific products and can be minimized by constant cooling during PCR setup. It is completely normal that in negative samples, for example, the negative control (NC), the primer dimer can be higher ...

• How to interpret the PCR results?

  Amplicons can either be analyzed by conventional gel electrophoresis with bands displaying at the size of the amplicons (460bp for the Mastermix 16S Complete and 310bp for the Mastermix 18S Complete). Alternatively, a real-time PCR cycler can be ...

• How to interpret the PCR results?

  PCR results Interpretation Amplicons can either be analyzed by conventional gel electrophoresis with bands displaying the size of the amplicons (460bp for the MA Bac and 310bp for the MA Yeasts). Alternatively, a real-time PCR cycler can be used, and ...

• How many PCR reactions are needed per patient?

  PCR Setup / PCR reactions per sample Every sample is applied to 3 (three) master mixes: MA Bac for 16S, MA Yeasts for 18S, and MA Control for the extraction control. All master mixes need at least one negative control and MA Bac and MA Yeasts have ...

• Can I use Molzym's PCR assays for quantitative PCR (qPCR) analysis?

  Products: Mastermix 16S Complete, Mastermix 18S Complete, PCR assays incl. in SepsiTest™-UMD, Micro-Dx™ and UMD-SelectNA™ No. Due to the broad-range primers (16S/18S) used, potential primer dimer formation and unspecific annealling can occur, so we ...