Pan Fungal Sequencing Primer Manuals

• Related Articles

• Set of Eubacterial Sequencing Primer Manuals

  The latest versions of the following manuals are available for download below: Set of Eubacterial Sequencing Primer Manual (English)

• Can I detect Borreliella (Borrelia) using Molzym's PCR and sequencing primers?

  Amplification of Borreliella (Borrelia) with Molzym PCR primers (incl. in MA Bac and Mastermix 16S Complete) should work successfully to produce a 16S amplicon (i.e. positive/negative PCR). However, due to mismatches with our sequencing primers ...

• For which application do I need the Set of Eubacterial Sequencing Primers?

  Set of Eubacterial Sequencing Primers SeqGP16 & SeqGN16 The two primers are used in conjunction with Mastermix 16S Complete. Both primers bind to conserved sites within the 16S rRNA gene region amplified in the assays. Primer SeqGP16 is ...

• For what application do I need the Panfungal Sequencing Primers?

  The primer is used in conjunction with Mastermix 18S Complete. The primer SeqYeast18 binds to conserved sites within the 18S rRNA gene region amplified in the assays.

• Can the BigDye® Terminator Direct Cycle Sequencing Kit be used with Molzym's sequencing primers?

  No, for the BigDye® Terminator Direct Cycle sequencing kit so called "M13 sequences" are required which are not included in Molzym's sequences. Please use the BigDye® Terminator v3.1 Cycle Sequencing Kit acc. to the manual.