Is the MolYsis™ technology applicable to plant samples?
The
MolYsis™ technology has not been systematically tested for plant samples and,
taking the structural properties of plant cells into account, the successful
host DNA depletion of the plant DNA does not seem likely.
Related Articles
Is the MolYsis™ technology applicable for viruses/viral NA isolation?
The MolYsis™ technology has not been systematically tested for the isolation of viral NA.
Can heat inactivated samples be used with MolYsis™-based kits?
Heat inactivated samples were not validated with MolYsis™. There are many variables in heat inactivation procedures, e.g. temperature and duration applied, effect of heat inactivation considering sample integrity beforehand and species present. ...
Can QIAGEN PowerBead solution/tubes be used with MolYsis™?
No, the QIAGEN Powerbead solution (incl. pre-filled Powerbead Tubes) cannot be used with MolYsis™-based kits since the solution contains a compound initiating cell lysis. Please note: For any MolYsis™-based kits the microbial cells need to be intact!
What is MolYsis™?
MolYsis™ is a technology that lyses host (human or animal) cells and quantitatively depletes host DNA (and all other free DNA) by a DNase treatment. In the second step, intact microorganisms are lysed, and highly enriched microbial DNA is isolated. ...
What to do when there is no thermomixer in my lab for the MolYsis™-based kits?
MolYsis & Thermomixer The MolYsis™ technology requires certain temperatures, 37°C, 56°C, and 70°C. During the incubation, samples should be mixed well. No Thermomixer? If no thermomixer with a shaking function is available, a heating block could be ...