Is the MolYsis™ technology applicable to plant samples?
The
MolYsis™ technology has not been systematically tested for plant samples and,
taking the structural properties of plant cells into account, the successful
host DNA depletion of the plant DNA does not seem likely.
MolYsis & Thermomixer The MolYsis™ technology requires certain temperatures, 37°C, 56°C, and 70°C. During the incubation, samples should be mixed well. No Thermomixer? If no thermomixer with a shaking function is available, a heating block could be ...
MolYsis™ is a technology that lyses host (human or animal) cells and quantitatively depletes host DNA (and all other free DNA) by a DNase treatment. In the second step, intact microorganisms are lysed, and highly enriched microbial DNA is isolated. ...
Please refer to the table below to see all differences: Kit name / specifications Sample materials & volumes Processing Host DNA depletion & lysis of bacterial and fungal cells Bacterial and Fungal DNA isolation MolYsis™ Basic5 Fluids < 1ml and 5 ml ...
RNase Please note that the MolYsis™-based kits are not systematically tested for the presence of RNases. After MolYsis™ Basic5 or step 7 of the other MolYsis™-based kits’ protocols, RNA isolation protocols could be followed.