Is the MolYsis™ technology applicable for viruses/viral NA isolation?

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• Is the MolYsis™ technology applicable to plant samples?

  The MolYsis™ technology has not been systematically tested for plant samples and, taking the structural properties of plant cells into account, the successful host DNA depletion of the plant DNA does not seem likely.

• After host DNA depletion with the MolYsis™ technology, can I isolate RNA?

  RNase Please note that the MolYsis™-based kits are not systematically tested for the presence of RNases. After MolYsis™ Basic5 or step 7 of the other MolYsis™-based kits’ protocols, RNA isolation protocols could be followed.

• What are the differences between the kits including the MolYsis™ technology?

  Please refer to the table below to see all differences: Kit name / specifications Sample materials & volumes Processing Host DNA depletion & lysis of bacterial and fungal cells Bacterial and Fungal DNA isolation MolYsis™ Basic5 Fluids < 1ml and 5 ml ...

• Can blood cultures be used for the isolation of microbial DNA?

  Blood culture All kits based on the MolYsis™ technology can be used for the isolation of microbial DNA from blood cultures. The blood culture samples shall be diluted at least 1:10 (100 µl blood culture + 900 µl SU buffer) before starting the ...

• What is MolYsis™?

  MolYsis™ is a technology that lyses host (human or animal) cells and quantitatively depletes host DNA (and all other free DNA) by a DNase treatment. In the second step, intact microorganisms are lysed, and highly enriched microbial DNA is isolated. ...