Interesting Publications
803 clinical specimens (e.g., biopsies, swabs, soft tissues, CSF) were compared using automated DNA extraction and broad-range
PCR (Micro-Dx™), standard culture, and enrichment culture procedures.
Shows the diagnostic value and subsequent adjustment of antimicrobial
treatment by using Micro-Dx™ on 529 culture-negative samples from normally
sterile body sites.
Related Articles
Why is broad-range 16S rDNA PCR a good option for the detection of bacteria?
Broad-range 16S rDNA PCR is a favorable way of detecting bacteria in samples due to two reasons. First, the use of highly conserved regions in the 16S rRNA gene allows the amplification of sequences from any bacterial strain. Mastermix 16S Complete ...Interesting Publications
Gyarmati et al. (2015): A study with 130 blood samples from hematological patients receiving dose-intensive antitumoral treatment were analyzed using MolYsis™ Complete5 and NGS sequencing. Link Thoendel et al. (2016): Previously negative sonicate ...Can I use Molzym's PCR assays for quantitative PCR (qPCR) analysis?
Products: Mastermix 16S Complete, Mastermix 18S Complete, PCR assays incl. in SepsiTest™-UMD, Micro-Dx™ and UMD-SelectNA™ No. Due to the broad-range primers (16S/18S) used, potential primer dimer formation and unspecific annealling can occur, so we ...Can blood culture samples be used?
Blood culture samples Yes, blood culture samples can be used but they first need to be diluted for use with SepsiTest™-UMD and Micro-Dx™. Please use 100 µl blood culture and 900 µl SU buffer for both kits. For Micro-Dx™, please ensure that the medium ...How to interpret the PCR results?
PCR results Interpretation Amplicons can either be analyzed by conventional gel electrophoresis with bands displaying the size of the amplicons (460bp for the MA Bac and 310bp for the MA Yeasts). Alternatively, a real-time PCR cycler can be used, and ...