How to process tissue?

Tissue Pre-treatment

Tissue is pre-treated with a 10-minute enzymatic approach followed by the normal protocol of host DNA depletion for SepsiTest™-UMD or placed into the SelectNA™plus instrument with Micro-Dx™. 

​Other tissue treatments like bead beating are not validated nor covered in the IVD workflow and possible disruption of the microbial cells or introduction of contaminations needs to be considered here.

Please note:

For SepsiTest™-UMD and Micro-Dx™ the microbial cells need to be intact!

Please refer to “What are the sample requirements?” for more details.

• Related Articles

• Can FFPE samples be analyzed with MolYsis™-based kits?

  FFPE Sample Yes, FFPE samples can be processed by simply following the tissue protocol of the Ultra-Deep Microbiome Prep and Ultra-Deep Microbiome Prep10 kits. There are no additional steps like deparaffinization required.

• What is MolYsis™?

  MolYsis™ is a technology that lyses host (human or animal) cells and quantitatively depletes host DNA (and all other free DNA) by a DNase treatment. In the second step, intact microorganisms are lysed, and highly enriched microbial DNA is isolated. ...

• Which sample sizes can be used?

  Clinical Sample Size & Volume For SepsiTest™-UMD and Micro-Dx™: Fluid samples: 1 ml. We recommend using as much sample volume as possible. For samples with smaller volumes, a buffer for volume adjustment to 1 ml is included in the kits. Tissue ...

• Can the SelectNA™plus instrument process any number of samples?

  Yes, the SelectNA™plus instrument can process any number of samples between 1 and 12. The sample number must be selected while loading the instrument for each run.

• How does the SelectNA™plus instrument work?

  SelectNAplus Process Process descriptions and details for the SelectNA™plus instrument are summarized in the technical information.