PCR results interpretation

How to interpret the PCR results?

  1. Amplicons can either be analyzed by conventional gel electrophoresis with bands displaying at the size of the amplicons (460bp for the Mastermix 16S Complete and 310bp for the Mastermix 18S Complete).
  2. Alternatively, a real-time PCR cycler can be used, and a melt curve analysis performed (please refer to the manual for the detailed PCR programs for various PCR cyclers).

Please
only evaluate the melt curve analysis for the interpretation of PCR results and not the amplification curves due to primer dimer formation.


 PCR Curve Interpretation (click attachment)