How to interpret PCR results?

How to interpret the PCR results?

PCR results Interpretation
  1. Amplicons can either be analyzed by conventional gel electrophoresis with bands displaying the size of the amplicons (460bp for the MA Bac and 310bp for the MA Yeasts).

  1. Alternatively, a real-time PCR cycler can be used, and a melt curve analysis performed (please refer to the manual for the detailed PCR programs for various PCR cyclers). Please only evaluate the melt curve analysis for the interpretation of PCR results and not the amplification curves

PCR Curve Interpretation (click on attachment)


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