How to purify PCR products?

How shall PCR products be purified?

We recommend the Qiagen QIAquick PCR purification kit (Qiagen, Germany) with an elution volume of 30µl.

Especially for samples with low microbial load, we achieved the best results with this kit.

We cannot recommend enzymatic purification methods or RapidTip PCR purification (Diffinity Genomics Inc., USA).


    • Related Articles

    • Can I use ExoSAP for PCR product clean-up?

      Molzym does not recommend ExoSAP for PCR product clean-up. Qiagen´s QIAquick® PCR Purification Kit (cat. no. 28104) has been validated for purification of the amplified PCR product produced by Molzym's kits. Although there are several published ...

    • Can I use Molzym's PCR assays for quantitative PCR (qPCR) analysis?

      Products: Mastermix 16S Complete, Mastermix 18S Complete, PCR assays incl. in SepsiTest™-UMD, Micro-Dx™ and UMD-SelectNA™ No. Due to the broad-range primers (16S/18S) used, potential primer dimer formation and unspecific annealling can occur, so we ...

    • How can bacteria and fungi be identified when detected by 16S or 18S rDNA PCR?

      Identification from positive amplicons The direct way is (Sanger) sequence analysis. Amplicons are purified in a simple procedure, using a commercial PCR purification kit (e.g. QIAQuick PCR Purification Kit, Qiagen, Germany). The purified amplicon is ...

    • Which real-time PCR cyclers are validated for SepsiTest™-UMD and Micro-Dx™?

      PCR Cyclers The following cyclers are validated: LightCycler® 1.5, 2.0, 96, 480 and Nano, Roche DNA Engine Opticon®, CFX96™, BioRad Mx3000P®, Mx3005P®, Stratagene ABI 7500 Fast®, Life Technologies Rotor-Gene®, Qiagen peqStar 96Q, peqlab Not suitable ...

    • MicroDx™ CE IVD Product Flyers

      The latest PDF versions of the following Flyers are available for download below: MicroDx™ CE IVD (English) a brief introduction to product features and visual overview of workflow and applications MicroDx™ CE IVD (German) eine kurze Einführung in ...