How much does a RipSeq® analysis cost for a mixed sequence?

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• How much does a RipSeq analysis cost for a mixed sequence?

  Please inquire about pricing from Pathogenomix directly.

• Can pathogens be identified in mixed infections? What to do with overlapping sequences?

  Polymicrobial infection Yes, for a fee, there is an online tool available from Pathogenomix called RipSeq® which can identify up to three bacterial species from a sequence depending on the sequence quality. With the two sequencing primers, SeqGN16 ...

• What is the approximate cost that 3rd party would charge to provide Sequencing services?

  Sanger Sequencing Service The cost for Sanger sequencing at a 3rd party is significantly below 10€ in Germany but might differ between countries and providers.

• Can pathogens be identified in mixed infections? What to do with overlapping sequences?

  Overlapping sequences are the result of several pathogens in the sample and, in the case of bacteria, could not be resolved by the sequencing primers SeqGN16 and SeqGP16. We recommend using an online tool available from Pathogenomix called RipSeq ...

• Can the BigDye® Terminator Direct Cycle Sequencing Kit be used with Molzym's sequencing primers?

  No, for the BigDye® Terminator Direct Cycle sequencing kit so called "M13 sequences" are required which are not included in Molzym's sequences. Please use the BigDye® Terminator v3.1 Cycle Sequencing kit acc. to the manual.