How is the human DNA depleted?
MolYsis Technology for human DNA depletion
- Samples are treated with a buffer
lysing the human cells, while bacterial and fungal cells stay intact.
- The
released human DNA and other free DNA are enzymatically degraded.
- Afterward, the microbial cells are lysed, and the DNA is purified and isolated.
- ​Please find a detailed description here: MolYsis™ technology.
Related Articles
How is human DNA depleted during sample processing?
Human DNA depletion Samples are treated with a buffer lysing the human/animal cells, while bacterial and fungal cells stay intact. The released human/animal DNA is enzymatically degraded along with all other free DNA and bacterial and fungal cells ...What is MolYsis™?
MolYsis™ is a technology that lyses host (human or animal) cells and quantitatively depletes host DNA (and all other free DNA) by a DNase treatment. In the second step, intact microorganisms are lysed, and highly enriched microbial DNA is isolated. ...Do I need to change my validated DNA purification system?
Not necessarily. Thanks to its modular nature, MolYsis™ Basic5 is adaptable to any DNA purification system. The kit is designed to treat <1 ml and 5 ml fluid samples including host DNA depletion and lysis of bacterial and fungal cells. Afterward, the ...What are the differences between the kits including the MolYsis™ technology?
Please refer to the table below to see all differences: Kit name / specifications Sample materials & volumes Processing Host DNA depletion & lysis of bacterial and fungal cells Bacterial and Fungal DNA isolation MolYsis™ Basic5 Fluids < 1ml and 5 ml ...After host DNA depletion with the MolYsis™ technology, can I isolate RNA?
RNase Please note that the MolYsis™-based kits are not systematically tested for the presence of RNases. After MolYsis™ Basic5 or step 7 of the other MolYsis™-based kits’ protocols, RNA isolation protocols could be followed.