How can the eluted DNA concentration be measured?
Diverse approaches are possible, but
please be aware that due to the depletion of human/animal DNA, the DNA
concentration might be quite low (between ng and fg amounts of DNA are isolated
depending on the sample used).
Possible methods include:
- qPCR
- Qubit
- Nanodrop
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Which applications are possible with the eluted DNA (after MolYsis™ technology)?
The eluted DNA can be used for diverse analyses For example: broad-range PCR and Sanger sequencing, specific PCR assays, hybridization assays, multiplex PCR, panel systems, custom PCR assay, NGS metagenomic/shotgun sequencing, whole genome ...Is the MolYsis™ technology applicable to plant samples?
The MolYsis™ technology has not been systematically tested for plant samples and, taking the structural properties of plant cells into account, the successful host DNA depletion of the plant DNA does not seem likely.After host DNA depletion with the MolYsis™ technology, can I isolate RNA?
RNase Please note that the MolYsis™-based kits are not systematically tested for the presence of RNases. After MolYsis™ Basic5 or step 7 of the other MolYsis™-based kits’ protocols, RNA isolation protocols could be followed.What are the differences between the kits including the MolYsis™ technology?
Please refer to the table below to see all differences: Kit name / specifications Sample materials & volumes Processing Host DNA depletion & lysis of bacterial and fungal cells Bacterial and Fungal DNA isolation MolYsis™ Basic5 Fluids < 1ml and 5 ml ...Is the MolYsis™ technology applicable for viruses/viral NA isolation?
The MolYsis™ technology has not been systematically tested for the isolation of viral NA.