How can bacteria and fungi be identified when detected by 16S or 18S rDNA PCR?
- Identification from positive amplicons
The direct way is (Sanger)
sequence analysis.
Amplicons are purified in a simple procedure, using a
commercial PCR purification kit (e.g. QIAQuick PCR Purification Kit,
Qiagen, Germany).
The purified amplicon is then included in a Sanger
sequencing reaction.
Sequencing primers are available matching the Mastermix 16S Complete and Mastermix 18S Complete.
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