Does Molzym manufacture customized mastermixes?
- Customized Mastermix
Yes. Please find further
information here and send inquiries on
customized manufactured master mixes to info@molzym.com
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What are the differences between the DNA-free master mixes and assays?
The assays Mastermix 16S Complete and Mastermix 18S Complete include already primers allowing for the ultra-sensitive detection of bacterial DNA (V3/V4 variable region of the 16S rRNA gene) and fungal DNA (V8/V9 variable region of the 18S rRNA gene) ...Can I use Molzym's PCR assays for quantitative PCR (qPCR) analysis?
Products: Mastermix 16S Complete, Mastermix 18S Complete, PCR assays incl. in SepsiTest™-UMD, Micro-Dx™ and UMD-SelectNA™ No. Due to the broad-range primers (16S/18S) used, potential primer dimer formation and unspecific annealling can occur, so we ...Can MolTaq 16S/18S and master mixes be used for Real-Time PCR?
MolTaq 16S/18S & real-time PCR Yes. All DNA-free PCR products of Molzym are fully compatible and optimized for Real-Time PCR, using fluorescent dye or probe detection systems.How many PCR reactions are needed per patient?
PCR Setup / PCR reactions per sample Every sample is applied to 3 (three) master mixes: MA Bac for 16S, MA Yeasts for 18S, and MA Control for the extraction control. All master mixes need at least one negative control and MA Bac and MA Yeasts have ...How can bacteria and fungi be identified when detected by 16S or 18S rDNA PCR?
Identification from positive amplicons The direct way is (Sanger) sequence analysis. Amplicons are purified in a simple procedure, using a commercial PCR purification kit (e.g. QIAQuick PCR Purification Kit, Qiagen, Germany). The purified amplicon is ...