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How many PCR reactions are needed per patient?
PCR Setup / PCR reactions per sample Every sample is applied to 3 (three) master mixes: MA Bac for 16S, MA Yeasts for 18S, and MA Control for the extraction control. All master mixes need at least one negative control and MA Bac and MA Yeasts have ...
How to interpret the PCR results?
PCR results Interpretation Amplicons can either be analyzed by conventional gel electrophoresis with bands displaying the size of the amplicons (460bp for the MA Bac and 310bp for the MA Yeasts). Alternatively, a real-time PCR cycler can be used, and ...
Which real-time PCR cyclers are validated for SepsiTest™-UMD and Micro-Dx™?
PCR Cyclers The following cyclers are validated: LightCycler® 1.5, 2.0, 96, 480 and Nano, Roche DNA Engine Opticon®, CFX96™, BioRad Mx3000P®, Mx3005P®, Stratagene ABI 7500 Fast®, Life Technologies Rotor-Gene®, Qiagen peqStar 96Q, peqlab Not suitable ...
The melt curve analysis shows a high primer dimer formation: why and what can I do?
Primer dimer Primer dimer formations are non-specific products and can be minimized by constant cooling during PCR setup. It is completely normal that in negative samples, for example, the negative control (NC), the primer dimer can be higher ...
Can I use Molzym's PCR assays for quantitative PCR (qPCR) analysis?
Products: Mastermix 16S Complete, Mastermix 18S Complete, PCR assays incl. in SepsiTest™-UMD, Micro-Dx™ and UMD-SelectNA™ No. Due to the broad-range primers (16S/18S) used, potential primer dimer formation and unspecific annealling can occur, so we ...