Can the BigDye® Terminator Direct Cycle Sequencing Kit be used with Molzym's sequencing primers?
Can the BigDye® Terminator Direct Cycle Sequencing Kit be used with Molzym's sequencing primers?
No, for the
BigDye® Terminator Direct Cycle sequencing kit
so called "M13 sequences" are required which are not included in Molzym's sequences.
Please use the
BigDye® Terminator v3.1 Cycle Sequencing kit
acc. to the manual.