Can pathogens be identified in mixed infections? What to do with overlapping sequences?
Polymicrobial infection
- Yes,
for a fee, there is an online tool available from Pathogenomix called RipSeq® which can identify up to three
bacterial species from a sequence depending on the sequence quality.
- With
the two sequencing primers, SeqGN16 and SeqGP16, up to six bacterial
species can theoretically be identified from one sample.
- RipSeq® is only suitable for mixed bacterial
sequences and cannot be used for yeasts and fungi.
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Can pathogens be identified in mixed infections? What to do with overlapping sequences?
Overlapping sequences are the result of several pathogens in the sample and, in the case of bacteria, could not be resolved by the sequencing primers SeqGN16 and SeqGP16. We recommend using an online tool available from Pathogenomix called RipSeq ...
What are the benefits of SepsiTest™ BLAST?
SepsiTest BLAST SepsiTest™ BLAST is a quality-controlled database with: 7,042 full-length 16S rRNA gene sequences, exclusively from cultivated reference strains of the described species of the domains Bacteria and Archaea 342 selected 18S rRNA gene ...
How can bacteria and fungi be identified when detected by 16S or 18S rDNA PCR?
Identification from positive amplicons The direct way is (Sanger) sequence analysis. Amplicons are purified in a simple procedure, using a commercial PCR purification kit (e.g. QIAQuick PCR Purification Kit, Qiagen, Germany). The purified amplicon is ...
How many sequencing reactions are needed per sample?
Sequencing For every sample with a positive 16S PCR, there are two sequencing reactions based on two sequencing primers, SeqGN16 and SeqGP16. This allows the identification of up to two pathogens, mainly separated into Gram-negative and Gram-positive ...
How much does a RipSeq® analysis cost for a mixed sequence?
Please inquire about pricing from Pathogenomix directly as availability and price may differ from country to country.