Can I detect Borreliella (Borrelia) using Molzym's PCR and sequencing primers?

• Related Articles

• For which application do I need the Set of Eubacterial Sequencing Primers?

  Set of Eubacterial Sequencing Primers SeqGP16 & SeqGN16 The two primers are used in conjunction with Mastermix 16S Complete. Both primers bind to conserved sites within the 16S rRNA gene region amplified in the assays. Primer SeqGP16 is ...

• Can I use ExoSAP for PCR product clean-up?

  Molzym does not recommend ExoSAP for PCR product clean-up. Qiagen´s QIAquick® PCR Purification Kit (cat. no. 28104) has been validated for purification of the amplified PCR product produced by Molzym's kits. Although there are several published ...

• How can bacteria and fungi be identified when detected by 16S or 18S rDNA PCR?

  Identification from positive amplicons The direct way is (Sanger) sequence analysis. Amplicons are purified in a simple procedure, using a commercial PCR purification kit (e.g. QIAQuick PCR Purification Kit, Qiagen, Germany). The purified amplicon is ...

• What is the approximate cost that 3rd party would charge to provide Sequencing services?

  Sanger Sequencing Service The cost for Sanger sequencing at a 3rd party is significantly below 10€ in Germany but might differ between countries and providers.

• Can I use Molzym's PCR assays for quantitative PCR (qPCR) analysis?

  Products: Mastermix 16S Complete, Mastermix 18S Complete, PCR assays incl. in SepsiTest™-UMD, Micro-Dx™ and UMD-SelectNA™ No. Due to the broad-range primers (16S/18S) used, potential primer dimer formation and unspecific annealling can occur, so we ...