Can heat inactivated samples be used with MolYsis™-based kits?

Heat inactivated samples were not validated with MolYsis™.

There are many variables in heat inactivation procedures, e.g. temperature and duration applied, effect of heat inactivation considering sample integrity beforehand and species present. Therefore, the results might be influenced in a way that we cannot recommend to use heat-inactivated samples.

Any damages to the cell walls of bacteria and/or fungi, will lead to a loss of microbial DNA during the host DNA depletion process resulting in a less sensitive or even false-negative analysis.

Please note: 

For any MolYsis™-based kits the microbial cells need to be intact!

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