Can FFPE samples be analyzed with MolYsis™-based kits?
FFPE Sample
Yes,
FFPE samples can be processed by simply following the tissue protocol of the
Ultra-Deep Microbiome Prep and Ultra-Deep Microbiome Prep10 kits.
There are no
additional steps like deparaffinization required.
Related Articles
What kind of samples can be processed with MolYsis™?
MolYsis™ has proven valuable for the analysis of a wide range of sample materials including various tissue specimens, body fluids, and swabs, especially for applications in the field of NGS and PCR based detection methods. Samples evaluated for ...
Ultra-Deep Microbiome Prep & Ultra-Deep Microbiome Prep10 RuO Manuals
The latest versions of the following manuals are available for download below: Ultra-Deep Microbiome Prep RuO Manual (English) Ultra-Deep Microbiome Prep RuO Short Manual (English) Ultra-Deep Microbiome Prep10 RuO Manual (English) Ultra-Deep ...
What to do when there is no centrifuge in my lab that can reach 9,500xg for 50 ml tubes (related to MolYsis™ Basic5, MolYsis™ Complete5, or Ultra-Deep Microbiome Prep10 kit)?
No centrifuge for 9500xg/50ml tubes If your lab is not equipped with a centrifuge that can reach 9500xg for 50ml tubes*, you can use a lower speed (e.g., 4,500xg) and should extend the centrifugation time to 20 min instead of the 10 min in the ...
Can I change the elution volume?
For all manual RUO kits (MolYsis™ Basic5, MolYsis™ Complete5, Ultra-Deep Microbiome Prep, and Ultra-Deep Microbiome Prep10) the elution volume might be changed. A change of the elution volume is not possible with the automated MolYsis-SelectNA™plus ...
Interesting Publications
Gyarmati et al. (2015): A study with 130 blood samples from hematological patients receiving dose-intensive antitumoral treatment were analyzed using MolYsis™ Complete5 and NGS sequencing. Link Thoendel et al. (2016): Previously negative sonicate ...