Can FFPE samples be used with MolYsis™-based kits?
FFPE Sample
Yes, formalin-fixed paraffin-embedded (FFPE) samples can be processed by simply following the tissue protocol of the
Ultra-Deep Microbiome Prep and Ultra-Deep Microbiome Prep10 kits.
There are no
additional steps like deparaffinization required.
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What kind of samples can be processed with MolYsis™?
MolYsis™ has proven valuable for the analysis of a wide range of sample materials including various tissue specimens, body fluids, and swabs, especially for applications in the field of NGS and PCR based detection methods. Samples evaluated for ...Ultra-Deep Microbiome Prep & Ultra-Deep Microbiome Prep10 RuO Manuals
The latest versions of the following manuals are available for download below: Ultra-Deep Microbiome Prep RuO Manual (English) Ultra-Deep Microbiome Prep RuO Short Manual (English) Ultra-Deep Microbiome Prep10 RuO Manual (English) Ultra-Deep ...What to do when there is no centrifuge in my lab that can reach 9,500xg for 50 ml tubes (related to MolYsis™ Basic5, MolYsis™ Complete5, or Ultra-Deep Microbiome Prep10 kit)?
No centrifuge for 9500xg/50ml tubes If your lab is not equipped with a centrifuge that can reach 9500xg for 50ml tubes*, you can use a lower speed (e.g., 4,500xg) and should extend the centrifugation time to 20 min instead of the 10 min in the ...Can I change the elution volume?
For all manual RUO kits (MolYsis™ Basic5, MolYsis™ Complete5, Ultra-Deep Microbiome Prep, and Ultra-Deep Microbiome Prep10) the elution volume might be changed. A change of the elution volume is not possible with the automated MolYsis-SelectNA™plus ...Interesting Publications
Gyarmati et al. (2015): A study with 130 blood samples from hematological patients receiving dose-intensive antitumoral treatment were analyzed using MolYsis™ Complete5 and NGS sequencing. Link Thoendel et al. (2016): Previously negative sonicate ...