Can blood cultures be used for the isolation of microbial DNA?
Blood culture
All kits based on the MolYsis™ technology can be used for the isolation of microbial DNA from blood cultures.
The blood
culture samples shall be diluted at least 1:10 (100 µl blood culture + 900 µl SU buffer) before starting the protocol.
In
a study, it was shown that bacterial growth in blood cultures can be detected
by PCR significantly earlier when using MolYsis™ Plus than the microbial
detection system (Gebert et al. 2008; DOI: 10.1016/j.jinf.2008.07.013).
Please
note: MolYsis™ Plus is not available anymore, please use MolYsis™ Complete 5
with a 1:10 dilution as described above.