Sanger Sequencing Service The cost for Sanger sequencing at a 3rd party is significantly below 10€ in Germany but might differ between countries and providers.
Sequencing For every sample with a positive 16S PCR, there are two sequencing reactions based on two sequencing primers, SeqGN16 and SeqGP16. This allows the identification of up to two pathogens, mainly separated into Gram-negative and Gram-positive ...
SepsiTest(tm)-UMD Micro-Dx(tm) Manual and short manual Yes Yes Reagents for sample pre-treatment e.g. tissue biopsies Yes Yes Reagents for host DNA depletion and microbial DNA isolation Buffers and enzymes Prefilled buffer cartridges and enzyme vials ...
PCR results Interpretation Amplicons can either be analyzed by conventional gel electrophoresis with bands displaying the size of the amplicons (460bp for the MA Bac and 310bp for the MA Yeasts). Alternatively, a real-time PCR cycler can be used, and ...
Amplification of Borreliella (Borrelia) with Molzym PCR primers (incl. in MA Bac and Mastermix 16S Complete) should work successfully to produce a 16S amplicon (i.e. positive/negative PCR). However, due to mismatches with our sequencing primers ...