Sanger Sequencing Service The cost for Sanger sequencing at a 3rd party is significantly below 10€ in Germany but might differ between countries and providers.
The latest PDF versions of the following Flyers are available for download below: MicroDx™ CE IVD (English) a brief introduction to product features and visual overview of workflow and applications MicroDx™ CE IVD (German) eine kurze Einführung in ...
Sequencing For every sample with a positive 16S PCR, there are two sequencing reactions based on two sequencing primers, SeqGN16 and SeqGP16. This allows the identification of up to two pathogens, mainly separated into Gram-negative and Gram-positive ...
SepsiTest(tm)-UMD Micro-Dx(tm) Manual and short manual Yes Yes Reagents for sample pre-treatment e.g. tissue biopsies Yes Yes Reagents for host DNA depletion and microbial DNA isolation Buffers and enzymes Prefilled buffer cartridges and enzyme vials ...
Amplification of Borreliella (Borrelia) with Molzym PCR primers (incl. in MA Bac and Mastermix 16S Complete) should work successfully to produce a 16S amplicon (i.e. positive/negative PCR). However, due to mismatches with our sequencing primers ...