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and a collection of frequently asked questions (FAQs).

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      • Popular Articles

      • Can FFPE samples be used with MolYsis™-based kits?

        FFPE Sample Yes, formalin-fixed paraffin-embedded (FFPE) samples can be processed by simply following the tissue protocol of the Ultra-Deep Microbiome Prep and Ultra-Deep Microbiome Prep10 kits. There are no additional steps like deparaffinization ...
      • What is included in the kits SepsiTest™-UMD and Micro-Dx™?

        SepsiTest(tm)-UMD Micro-Dx(tm) Manual and short manual Yes Yes Reagents for sample pre-treatment e.g. tissue biopsies Yes Yes Reagents for host DNA depletion and microbial DNA isolation Buffers and enzymes Prefilled buffer cartridges and enzyme vials ...
      • What are the sample requirements? How shall samples be stored after sample collection? How fresh or old can samples be?

        Please note: For any MolYsis™-based kits the microbial cells need to be intact! After taking the samples, please cool the samples in the fridge at ~8°C for short-term storage. Optimally, fresh samples are used for SepsiTest™-UMD and Micro-Dx™ for the ...
      • How is the human DNA depleted?

        MolYsis Technology for human DNA depletion Samples are treated with a buffer lysing the human cells, while bacterial and fungal cells stay intact. The released human DNA and other free DNA are enzymatically degraded. Afterward, the microbial cells ...
      • How many PCR reactions are needed per patient?

        PCR Setup / PCR reactions per sample Every sample is applied to 3 (three) master mixes: MA Bac for 16S, MA Yeasts for 18S, and MA Control for the extraction control. All master mixes need at least one negative control and MA Bac and MA Yeasts have ...